The 1910HK/RR two-component system is essential for the virulence of Streptococcus suis serotype 2

Fangyan Yuan; Chen Tan; Zewen Liu; Keli Yang; Danna Zhou; Wei Liu; Zhengying Duan; Rui Guo; Huanchun Chen; Yongxiang Tian; Weicheng Bei

doi:10.1016/j.micpath.2016.12.026

The 1910HK/RR two-component system is essential for the virulence of Streptococcus suis serotype 2

Microb Pathog. 2017 Mar:104:137-145. doi: 10.1016/j.micpath.2016.12.026. Epub 2017 Jan 14.

Authors

Fangyan Yuan¹, Chen Tan², Zewen Liu³, Keli Yang³, Danna Zhou³, Wei Liu³, Zhengying Duan³, Rui Guo³, Huanchun Chen², Yongxiang Tian³, Weicheng Bei⁴

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
² State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China.
³ Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430070, China.
⁴ State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: beiwc@mail.hzau.edu.cn.

PMID: 28093234
DOI: 10.1016/j.micpath.2016.12.026

Abstract

Streptococcus suis serotype 2 is a major zoonotic pathogen, and the two-component system plays an important role in bacterial pathogenesis. The present study targeted the 1910HK/RR two-component system of S. suis 2. A 1910HK/RR deletion mutant (Δ1910HK/RR) and the corresponding complementation strain (CΔ1910HK/RR) were constructed in S. suis 2 strain 05ZYH33. 1910HK/RR deletion had no effect on S. suis 2 growth, but significantly inhibited the adherence and invasion of S. suis 2 to HEp-2 cells. Analysis of the role of 1910HK/RR in murine and pig infection models demonstrated that 1910HK/RR played a distinct role in the virulence of S. suis 2. In addition, deletion of 1910HK/RR significantly impaired the survival of 05ZYH33 in human blood. These data provided important insights into the pathogenesis of S. suis 2.

Keywords: 1910HK/RR; Streptococcus suis; Two-component system; Virulence.

MeSH terms

Animals
Bacterial Adhesion / genetics
Cell Line
Cells, Cultured
Disease Models, Animal
Female
Gene Knockout Techniques
Gene Order
Genetic Complementation Test
Genetic Loci
Genome, Bacterial
Genomic Islands*
Humans
Mice
Microbial Viability
Sequence Deletion
Serogroup
Streptococcal Infections / microbiology*
Streptococcal Infections / mortality
Streptococcal Infections / pathology
Streptococcus suis / classification
Streptococcus suis / genetics*
Streptococcus suis / growth & development
Streptococcus suis / pathogenicity*
Swine
Virulence / genetics