A development and an improvement of selectable markers in Pleurotus ostreatus transformation

Yukari Matsunaga; Megumi Ando; Kosuke Izumitsu; Kazumi Suzuki; Yoichi Honda; Toshikazu Irie

doi:10.1016/j.mimet.2017.01.007

A development and an improvement of selectable markers in Pleurotus ostreatus transformation

J Microbiol Methods. 2017 Mar:134:27-29. doi: 10.1016/j.mimet.2017.01.007. Epub 2017 Jan 10.

Authors

Yukari Matsunaga¹, Megumi Ando¹, Kosuke Izumitsu¹, Kazumi Suzuki¹, Yoichi Honda², Toshikazu Irie³

Affiliations

¹ School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan.
² Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto Prefecture 606-8502, Japan.
³ School of Environmental Science, University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga 522-8533, Japan. Electronic address: tirie@ses.usp.ac.jp.

PMID: 28087470
DOI: 10.1016/j.mimet.2017.01.007

Abstract

Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3±0.6transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7±11.5transformants/μg plasmid DNA.

Keywords: Basidiomycete; Mushroom; White-rot.

MeSH terms

Antifungal Agents / pharmacology
DNA, Fungal / genetics*
Drug Resistance, Fungal / genetics
Genetic Markers
Organophosphorus Compounds / pharmacology
Plasmids
Pleurotus / drug effects
Pleurotus / genetics*
Streptothricins / pharmacology
Transformation, Genetic*

Substances

Antifungal Agents
DNA, Fungal
Genetic Markers
Organophosphorus Compounds
bialaphos
Streptothricins