In Vitro Gene Delivery with Large Porous Silicon Nanoparticles Fabricated Using Cost-Effective, Metal-Assisted Chemical Etching

Nancy Wareing; Kyle Szymanski; Giridhar R Akkaraju; Armando Loni; Leigh T Canham; Roberto Gonzalez-Rodriguez; Jeffery L Coffer

doi:10.1002/smll.201602739

In Vitro Gene Delivery with Large Porous Silicon Nanoparticles Fabricated Using Cost-Effective, Metal-Assisted Chemical Etching

Small. 2017 Jan;13(3). doi: 10.1002/smll.201602739. Epub 2016 Nov 7.

Authors

Nancy Wareing¹, Kyle Szymanski¹, Giridhar R Akkaraju¹, Armando Loni², Leigh T Canham², Roberto Gonzalez-Rodriguez³, Jeffery L Coffer³

Affiliations

¹ Department of Biology, Texas Christian University, Fort Worth, TX, 76129, USA.
² pSiMedica Ltd, Malvern Hills Science Park, Geraldine Road, Malvern, Worcestershire, WR14 3 SZ, UK.
³ Department of Chemistry, Texas Christian University, Fort Worth, TX, 76129, USA.

PMID: 28084695
DOI: 10.1002/smll.201602739

Abstract

The cytocompatibility, cell membrane affinity, and plasmid DNA delivery from surface oxidized, metal-assisted stain-etched mesoporous silicon nanoscale particles (pSiNPs) to human embryonic kidney (HEK293) cells is demonstrated, suggesting the possibility of using such material for targeted transfection and drug delivery.

Keywords: gene delivery; nanomedicine; porous silicon; stain etching; surface modification.

MeSH terms

Cost-Benefit Analysis
Fluorescein-5-isothiocyanate
Gene Transfer Techniques*
HEK293 Cells
Humans
Metals / chemistry*
Microscopy, Confocal
Nanoparticles / chemistry*
Particle Size
Porosity
Silicon / chemistry*
Sonication

Substances

Metals
Fluorescein-5-isothiocyanate
Silicon