An ESIPT-Based Fluorescent Probe for Hydrazine Detection in Aqueous Solution and its Application in Living Cells

Zhiyu Ju; Dapeng Li; Di Zhang; Dandan Li; Changzeng Wu; Zhihong Xu

doi:10.1007/s10895-016-1997-7

An ESIPT-Based Fluorescent Probe for Hydrazine Detection in Aqueous Solution and its Application in Living Cells

J Fluoresc. 2017 Mar;27(2):679-687. doi: 10.1007/s10895-016-1997-7. Epub 2017 Jan 13.

Authors

Zhiyu Ju¹, Dapeng Li², Di Zhang³, Dandan Li², Changzeng Wu², Zhihong Xu⁴

Affiliations

¹ College of Chemistry and Chemical Engineering, Xuchang University, Xuchang, 461000, China. juz1696@163.com.
² College of Chemistry and Chemical Engineering, Xuchang University, Xuchang, 461000, China.
³ Institute of Quality Standards and Testing Technology for Agro-Products, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
⁴ College of Chemistry and Chemical Engineering, Xuchang University, Xuchang, 461000, China. xuzhihong1980@yahoo.com.

PMID: 28084555
DOI: 10.1007/s10895-016-1997-7

Abstract

A new fluorescent probe based on "protection-deprotection" sequence and the excited-state intramolecular proton transfer (ESIPT) effect was designed and synthesized. The probe responds rapidly and high selectively toward hydrazine at room temperature, and exhibits a low detection limit of 0.016 μM (0.512 ppb), which is well below the safety level (10 ppb) as regulated by EPA. Moreover, it can be applied for the imaging of hydrazine in living cells.

Keywords: Biological imaging; Excited state intramolecular proton transfer; Fluorescence; Hydrazine.

MeSH terms

Esophageal Neoplasms / metabolism*
Fluorescent Dyes / chemistry*
Humans
Hydrazines / analysis*
Protons*
Spectrometry, Fluorescence / methods*
Tumor Cells, Cultured
Water / analysis*

Substances

Fluorescent Dyes
Hydrazines
Protons
Water
hydrazine