De novo AML exhibits greater microenvironment dysregulation compared to AML with myelodysplasia-related changes

Sci Rep. 2017 Jan 13:7:40707. doi: 10.1038/srep40707.

Abstract

The interaction between the bone marrow microenvironment and malignant hematopoietic cells can result in the protection of leukemia cells from chemotherapy in both myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). We, herein, characterized the changes in cytokine expression and the function of mesenchymal stromal cells (MSC) in patients with MDS, AML with myelodysplasia-related changes (MRC), a well-recognized clinical subtype of secondary AML, and de novo AML. We observed a significant inhibitory effect of MDS-MSC on T lymphocyte proliferation and no significant differences in any of the cytokines tested. AML-MSC inhibited T-cell proliferation only at a very low MSC/T cell ratio. When compared to the control, AML-MRCderived MSC presented a significant increase in IL6 expression, whereas de novo AML MSC presented a significant increase in the expression levels of VEGFA, CXCL12, RPGE2, IDO, IL1β, IL6 and IL32, followed by a decrease in IL10 expression. Furthermore, data indicate that IL-32 regulates stromal cell proliferation, has a chemotactic potential and participates in stromal cell crosstalk with leukemia cells, which could result in chemoresistance. Our results suggest that the differences between AML-MRC and de novo AML also extend into the leukemic stem cell niche and that IL-32 can participate in the regulation of the bone marrow cytokine milieu.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antimetabolites, Antineoplastic / pharmacology
  • Bone Marrow / pathology
  • Bone Marrow Cells / metabolism
  • Bone Marrow Cells / pathology
  • Case-Control Studies
  • Cell Line
  • Cell Proliferation
  • Cellular Microenvironment* / drug effects
  • Cellular Microenvironment* / genetics
  • Cellular Microenvironment* / immunology
  • Chemotaxis / genetics
  • Chemotaxis / immunology
  • Cytarabine / pharmacology
  • Cytokines / metabolism
  • Female
  • Gene Expression Regulation
  • Gene Silencing
  • Humans
  • Immunomodulation
  • Leukemia, Myeloid, Acute / etiology*
  • Leukemia, Myeloid, Acute / metabolism*
  • Leukemia, Myeloid, Acute / pathology
  • Male
  • MicroRNAs / genetics
  • Mitogen-Activated Protein Kinases / metabolism
  • Myelodysplastic Syndromes / etiology*
  • Myelodysplastic Syndromes / metabolism*
  • Myelodysplastic Syndromes / pathology
  • NF-kappa B / metabolism
  • RNA Interference
  • Signal Transduction
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocyte Subsets / metabolism
  • Tumor Microenvironment* / drug effects
  • Tumor Microenvironment* / genetics
  • Tumor Microenvironment* / immunology

Substances

  • Antimetabolites, Antineoplastic
  • Cytokines
  • MicroRNAs
  • NF-kappa B
  • Cytarabine
  • Mitogen-Activated Protein Kinases