Curculactones A and B induced the differentiation of C3H10T1/2 and MC3T3-E1 cells to osteoblasts

Bioorg Med Chem Lett. 2017 Mar 1;27(5):1301-1303. doi: 10.1016/j.bmcl.2016.12.070. Epub 2016 Dec 30.

Abstract

Curculactones A and B are rare γ-lactone derivatives obtained from yellow, natural curcumin following γ-irradiation, and are a type of small molecules with a moderate anti-obesity effect. However, the exact role of curculactones A and B in osteoblast differentiation is unknown. In this study, the effects of curculactones A and B on the differentiation of the mesenchymal cell line C3H10T1/2 and pre-osteoblast cell line MC3T3-E1 to osteoblasts were examined. Curculactones A or B could markedly increase the mRNA levels of osteogenic marker genes and alkaline phosphatase (ALP) activity. Collectively, our findings indicate that curculactones A or B induced osteoblast differentiation through osteogenic expression of genes such as distal-less homeobox 5 (Dlx5), runt-related transcription factor 2 (Runx2), ALP, and osteocalcin (OC).

Keywords: ALP; Bone; Curculactones A and B; Mesenchymal stem cells; Osteoblast differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation / drug effects*
  • Cell Line
  • Lactones / chemical synthesis
  • Lactones / chemistry
  • Lactones / pharmacology*
  • Mice
  • Molecular Structure
  • Osteoblasts / cytology*
  • Polymerase Chain Reaction

Substances

  • Lactones
  • curculactone A
  • curculactone B