CD95-Mediated Calcium Signaling

Methods Mol Biol. 2017:1557:79-93. doi: 10.1007/978-1-4939-6780-3_8.

Abstract

Intracellular calcium signals regulate cell function and cell survival by controlling many processes. CD95 engagement results in distinct intracellular calcium signals that control the cell fate, apoptosis, or survival, depending on the ligand (membrane or soluble). Intracellular calcium determination is an exquisite readout to explore the molecular mechanisms elicited by CD95 engagement. The most widely applied methods for studying calcium signaling pathways use fluorescent indicators and imaging methods with fluorescence microscopy. This technical approach, however, requires many precautions that we discuss in this chapter.

Keywords: Confocal microscopy; Fluo2; Fura2; MitoTracker; Mitochondria; Pluronic acid® F-127; Rhod2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism*
  • Calcium Signaling*
  • Cell Line
  • Fas Ligand Protein / metabolism
  • Humans
  • Microscopy, Confocal
  • Mitochondria / metabolism
  • Molecular Imaging / methods
  • Protein Binding
  • fas Receptor / metabolism*

Substances

  • Fas Ligand Protein
  • fas Receptor
  • Calcium