Eotaxin-2 increased toll-like receptor 4 expression in endothelial cells in vitro and exacerbates high-cholesterol diet-induced atherogenesis in vivo

Chien-Sung Tsai; Chun-Yao Huang; Chien-Ho Chen; Yi-Wen Lin; Chun-Ming Shih; Nai-Wen Tsao; Kuang-Hsing Chiang; Chi-Yuan Lee; Hellen Jeng; Feng-Yen Lin

Eotaxin-2 increased toll-like receptor 4 expression in endothelial cells in vitro and exacerbates high-cholesterol diet-induced atherogenesis in vivo

Am J Transl Res. 2016 Dec 15;8(12):5338-5353. eCollection 2016.

Authors

Affiliations

¹ Division of Cardiovascular Surgery, Department of Surgery, Taoyuan Armed Forces General HospitalTaoyuan, Taiwan; Division of Cardiovascular Surgery, Department of Surgery, Tri-Service General HospitalTaipei, Taiwan; Department and Graduate Institute of Pharmacology, National Defense Medical CenterTaipei, Taiwan.
² Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical UniversityTaipei, Taiwan; Division of Cardiology and Cardiovascular Research Center, Department of Internal Medicine, Taipei Medical University HospitalTaipei, Taiwan.
³ School of Medical Laboratory Science and Biotechnology Taipei Medical University Taipei, Taiwan.
⁴ Division of Cardiovascular Surgery, Department of Surgery, Tri-Service General HospitalTaipei, Taiwan; Institute of Oral Biology, National Yang-Ming UniversityTaipei, Taiwan.
⁵ Division of Cardiovascular Surgery, Taipei Medical University Hospital Taipei, Taiwan.
⁶ Graduate Institute of Clinical Medical Sciences, China Medical University Taichung, Taiwan.
⁷ Division of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University Taipei, Taiwan.

PMID: 28078007
PMCID: PMC5209487

Abstract

Eotaxin-2 is a potent chemoattractant. High concentration of eotaxin-2 triggers the inflammation and tumor metastasis. Inhibition of eotaxin-2 may protect experimental atherogenesis although the mechanism is still unclear. Toll-like receptor 4 (TLR4) plays a major role mediating vascular inflammation, which is related to atherogenesis. In the results, we demonstrated that eotaxin-2 potentially impairs the tube formation capacity of human coronary artery endothelial cells (HCAECs). Eotaxin-2 augments the monocytic adhesion in lipopolysaccharides (LPS)-induced HCAECs, and which were reversed by TLR4 siRNA. Thus this study was conducted to investigate whether eotaxin-2 increases TLR4 expression, and then enhances the sensitivity of cells to antigen stimulation in HCAECs, which mediates the increasing of the development of serious atherosclerosis. In fact, we showed that JNK/SAPK, p38 MAPK, and ERK1/2 activation contribute to the transcriptional signaling pathway, JNK/SAPK and p38 MAPK regulate post-transcriptional modification, as well as protein-trafficking pathway in eotaxin-2-treated HCAECs TLR4 expression. RNA binding proteins, such as human antigen R (HuR) and tristetraprolin (TTP) mediate stability of TLR4 mRNA and chaperone, such as PRAT4A (a protein associated with TLR4) regulate trafficking of TLR4 protein might confer eotaxin-2 responsiveness. Eotaxin-2 administration led to a significant elevation of high cholesterol diet-induced atherosclerosis, and of TLR4 expression in B6.129S7-Ldlr^tm1Her /J but not Ldlr^-/--/-/Tlr4^-/- mice. Our results revealed that eotaxin-2 induced overexpression TLR4 via mitogen-activated protein kinases (MAPK) signaling pathways, RNA binding proteins-mediated mRNA stabilization, and PRAT4A-regulated trafficking in HCAECs. These effects may lead to amplification of inflammatory responses contribute to the pathogenesis of cardiovascular disorders.

Keywords: Eotaxin-2; atherosclerosis; endothelial cells; toll-like receptor 4.