Nutrient recycle from defatted microalgae (Aurantiochytrium) with hydrothermal treatment for microalgae cultivation

Taku Michael Aida; Ryouma Maruta; Yuuhiko Tanabe; Minori Oshima; Toshiyuki Nonaka; Hiroki Kujiraoka; Yasuaki Kumagai; Masaki Ota; Iwane Suzuki; Makoto M Watanabe; Hiroshi Inomata; Richard L Smith Jr

doi:10.1016/j.biortech.2016.12.078

Nutrient recycle from defatted microalgae (Aurantiochytrium) with hydrothermal treatment for microalgae cultivation

Bioresour Technol. 2017 Mar:228:186-192. doi: 10.1016/j.biortech.2016.12.078. Epub 2016 Dec 24.

Authors

Affiliations

¹ Graduate School of Environmental Studies, Tohoku University, Aramaki Aza Aoba 6-6-11, Aoba-ku, Sendai 980-8579, Japan. Electronic address: aida@scf.che.tohoku.ac.jp.
² Graduate School of Environmental Studies, Tohoku University, Aramaki Aza Aoba 6-6-11, Aoba-ku, Sendai 980-8579, Japan.
³ Research Center of Supercritical Fluid Technology, Tohoku University, Aramaki Aza Aoba 6-6-11, Aoba-ku, Sendai 980-8579, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
⁴ Research Center of Supercritical Fluid Technology, Tohoku University, Aramaki Aza Aoba 6-6-11, Aoba-ku, Sendai 980-8579, Japan.
⁵ Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
⁶ Graduate School of Environmental Studies, Tohoku University, Aramaki Aza Aoba 6-6-11, Aoba-ku, Sendai 980-8579, Japan; Research Center of Supercritical Fluid Technology, Tohoku University, Aramaki Aza Aoba 6-6-11, Aoba-ku, Sendai 980-8579, Japan.

PMID: 28063361
DOI: 10.1016/j.biortech.2016.12.078

Abstract

Defatted heterotrophic microalgae (Aurantiochytrium limacinum SR21) was treated with high temperature water (175-350°C, 10-90min) to obtain nitrogen and phosphorous nutrients as a water soluble fraction (WS). Yields of nitrogen and phosphorous recovered in WS varied from 38 to 100% and from 57 to 99%, respectively. Maximum yields of nitrogen containing compounds in WS were proteins (43%), amino acids (12%) and ammonia (60%) at treatment temperatures of 175, 250 and 350°C, respectively. Maximum yield of phosphorous in WS was 99% at a treatment temperature of 250°C. Cultivation experiments of microalgae (A. limacinum SR21) using WS obtained at 200 and 250°C showed positive growth. Water soluble fractions from hydrothermal treatment of defatted microalgae are effective nitrogen and phosphorous nutrient sources for microalgae cultivation.

Keywords: Amino acid; Biosolid treatment process; Internal recycle; Phosphorous; Protein.

MeSH terms

Biomass
Food
Heterotrophic Processes
Hot Temperature
Microalgae / chemistry
Microalgae / growth & development*
Microalgae / metabolism*
Nitrogen / metabolism*
Phosphorus / metabolism*
Recycling
Stramenopiles / metabolism
Water / chemistry

Substances

Water
Phosphorus
Nitrogen