Diffusion tensor imaging measures of white matter compared to myelin basic protein immunofluorescence in tissue cleared intact brains

Eric H Chang; Miklos Argyelan; Manisha Aggarwal; Toni-Shay S Chandon; Katherine H Karlsgodt; Susumu Mori; Anil K Malhotra

doi:10.1016/j.dib.2016.12.018

Diffusion tensor imaging measures of white matter compared to myelin basic protein immunofluorescence in tissue cleared intact brains

Data Brief. 2016 Dec 18:10:438-443. doi: 10.1016/j.dib.2016.12.018. eCollection 2017 Feb.

Authors

Eric H Chang¹, Miklos Argyelan¹, Manisha Aggarwal², Toni-Shay S Chandon¹, Katherine H Karlsgodt³, Susumu Mori⁴, Anil K Malhotra³

Affiliations

¹ Center for Psychiatric Neuroscience, The Feinstein Institute for Medical Research, Northwell Health, 350 Community Drive, Manhasset, NY 11030, USA; Division of Psychiatry Research, Zucker Hillside Hospital, Northwell Health, 75-59 263rd Street, Glen Oaks, NY 11004, USA.
² Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
³ Center for Psychiatric Neuroscience, The Feinstein Institute for Medical Research, Northwell Health, 350 Community Drive, Manhasset, NY 11030, USA; Division of Psychiatry Research, Zucker Hillside Hospital, Northwell Health, 75-59 263rd Street, Glen Oaks, NY 11004, USA; Hofstra Northwell School of Medicine, Departments of Psychiatry and Molecular Medicine, Hofstra University, Hempstead, NY, USA.
⁴ Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD 21205, USA.

Abstract

We provide datasets from combined ex vivo diffusion tensor imaging (DTI) and Clear Lipid-exchanged, Anatomically Rigid, Imaging/immunostaining compatible, Tissue hYdrogel (CLARITY) performed on intact mouse brains. DTI-derived measures of fractional anisotropy (FA), radial diffusivity (RD), and axial diffusivity (AD) were compared to antibody-based labeling of myelin basic protein (MBP), as measured by fluorescence microscopy. We used a customized CLARITY hydrogel solution to facilitate whole brain tissue clearing and subsequent immunolabeling. We describe how CLARITY was made compatible with magnetic resonance imaging with the intention of facilitating future multimodal imaging studies that may combine noninvasive imaging with 3D immunohistochemistry. These data and methods are related to the accompanying research article entitled, 'The role of myelination in measures of white matter integrity: Combination of diffusion tensor imaging and two-photon microscopy of CLARITY intact brains' (E.H. Chang, M. Argyelan, M. Aggarwal, T-S. Chandon, K.H. Karlsgodt, S. Mori, A.K. Malhotra, 2016) [1].

Keywords: CLARITY; DTI; Multimodal imaging; Myelination; Radial diffusivity.

Grants and funding

P50 MH080173/MH/NIMH NIH HHS/United States