Evidence That Trimethyllysine Hydroxylase Catalyzes the Formation of (2S,3S)-3-Hydroxy-Nε-trimethyllysine

Y Vijayendar Reddy; Abbas H K Al Temimi; Paul B White; Jasmin Mecinović

doi:10.1021/acs.orglett.6b03608

Evidence That Trimethyllysine Hydroxylase Catalyzes the Formation of (2S,3S)-3-Hydroxy-N^ε-trimethyllysine

Org Lett. 2017 Jan 20;19(2):400-403. doi: 10.1021/acs.orglett.6b03608. Epub 2017 Jan 3.

Authors

Y Vijayendar Reddy¹, Abbas H K Al Temimi¹, Paul B White¹, Jasmin Mecinović¹

Affiliation

¹ Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.

PMID: 28045275
DOI: 10.1021/acs.orglett.6b03608

Abstract

Trimethyllysine hydroxylase (TMLH) is an Fe(II) and 2-oxoglutarate (2OG) dependent oxygenase involved in the biomedically important carnitine biosynthesis pathway. A combination of synthetic and NMR studies provides direct evidence that human TMLH catalyzes the stereoselective conversion of (2S)-N^ε-trimethyllysine to (2S,3S)-3-hydroxy-N^ε-trimethyllysine.

MeSH terms

Biocatalysis
Carnitine
Humans
Lysine / analogs & derivatives
Mixed Function Oxygenases / metabolism*
Molecular Structure

Substances

trimethyllysine
Mixed Function Oxygenases
trimethyl-lysine hydroxylase
Lysine
Carnitine