In silico structure analysis and epitope prediction of E3 CR1-beta protein of Human Adenovirus E for vaccine design

Noman Ibna Amin Patwary; Md Saiful Islam; Md Sohel; Ismot Ara; Mohd Omar Faruk Sikder; Shah Md Shahik

doi:10.1016/j.bj.2016.11.004

In silico structure analysis and epitope prediction of E3 CR1-beta protein of Human Adenovirus E for vaccine design

Biomed J. 2016 Dec;39(6):382-390. doi: 10.1016/j.bj.2016.11.004. Epub 2016 Dec 27.

Authors

Noman Ibna Amin Patwary¹, Md Saiful Islam¹, Md Sohel¹, Ismot Ara², Mohd Omar Faruk Sikder¹, Shah Md Shahik³

Affiliations

¹ Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Bangladesh.
² Department of Computer Science and Engineering, Faculty of Science and Technology, Atish Dipankar University of Science and Technology, Bangladesh; Department of Computer Science and Engineering, American International University-Bangladesh, Bangladesh.
³ Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Bangladesh. Electronic address: sm.shahik@gmail.com.

Abstract

Background: Human Adenoviruses are divided into 7 species of Human Adenovirus A to G based on DNA genome homology. The Human Adenovirus E (HAdVs-E) genome is a linear, double-stranded DNA containing 38 protein-coding genes. Wild-type adenoviruses type E, are linked to a number of slight illnesses. The most important part of HAdVs-E is E3 CR1-beta protein which controls the host immune response and viral attachment.

Method: We use numerous bio-informatics and immuno-informatics implements comprising sequence and construction tools for construction of 3D model and epitope prediction for HAdVs-E.

Results: The 3D structure of E3 CR1-beta protein was generated and total of ten antigenic B cell epitopes, 6 MHC class I and 11 MHC class II binding peptides were predicted.

Conclusion: The study was carried out to predict antigenic determinants/epitopes of the E3 CR1-beta protein of Human Adenovirus E along with the 3D protein modeling. The study revealed potential T-cell and B-cell epitopes that can raise the desired immune response against E3 CR1-beta protein and useful in developing effective vaccines against HAdVs-E.

Keywords: 3D structure prediction; B cell epitope; Human Adenoviruses E; T cell epitope; Vaccine.

MeSH terms

Adenovirus E3 Proteins / chemistry
Adenovirus E3 Proteins / immunology*
Adenoviruses, Human / immunology*
B-Lymphocytes / immunology*
Computer Simulation
Epitopes* / chemistry
Humans
Protein Binding
T-Lymphocytes / immunology*
Vaccines / chemistry*

Substances

Adenovirus E3 Proteins
Epitopes
Vaccines