A nematicidal tannin from Punica granatum L. rind and its physiological effect on pine wood nematode (Bursaphelenchus xylophilus)

Pestic Biochem Physiol. 2017 Jan:135:64-68. doi: 10.1016/j.pestbp.2016.06.003. Epub 2016 Jun 8.

Abstract

The ethanol extract of Punica granatum L. rind was tested to show significant nematicidal activity against pine wood nematode. Three nematicidal compounds were obtained from the ethanol extract by bioassay-guided fractionation and identified as punicalagin 1, punicalin 2, and corilagin 3 by mass and nuclear magnetic resonance spectral data analysis. Punicalagin 1 was most active against PWN among the purified compounds with the LC50 value of 307.08μM in 72h. According to the enzyme assays in vitro, punicalagin 1 could inhibit the activity of acetylcholinesterase, amylase and cellulase from PWN with IC50 value of 0.60mM, 0.96mM and 1.24mM, respectively. The morphological structures of PWNs treated by punicalagin 1 were greatly changed. These physiological effects of punicalagin 1 on PWN may helpful to elucidate its nematicidal mechanism.

Keywords: Bursaphelenchus xylophilus; Nematicidal activity; Physiological effect; Punica granatum L. rind; Punicalagin.

MeSH terms

  • Acetylcholinesterase / metabolism
  • Amylases / antagonists & inhibitors
  • Animals
  • Antinematodal Agents / chemistry
  • Antinematodal Agents / toxicity*
  • Cellulase / antagonists & inhibitors
  • Cholinesterase Inhibitors / chemistry
  • Cholinesterase Inhibitors / toxicity
  • Glucosides / analysis
  • Glucosides / toxicity
  • Hydrolyzable Tannins / analysis
  • Hydrolyzable Tannins / toxicity*
  • Lythraceae*
  • Microscopy, Electron, Scanning
  • Microscopy, Electron, Transmission
  • Plant Extracts / chemistry
  • Plant Extracts / toxicity*
  • Tylenchida / drug effects*
  • Tylenchida / enzymology
  • Tylenchida / ultrastructure

Substances

  • Antinematodal Agents
  • Cholinesterase Inhibitors
  • Glucosides
  • Hydrolyzable Tannins
  • Plant Extracts
  • corilagin
  • punicalagin
  • punicalin
  • Acetylcholinesterase
  • Amylases
  • Cellulase