Phyllanthus emblica seed extract mediated synthesis of PdNPs against antibacterial, heamolytic and cytotoxic studies

Murugesan Dinesh; Selvaraj Mohana Roopan; Chinnadurai Immanuel Selvaraj; Prabhakarn Arunachalam

doi:10.1016/j.jphotobiol.2016.12.012

Phyllanthus emblica seed extract mediated synthesis of PdNPs against antibacterial, heamolytic and cytotoxic studies

J Photochem Photobiol B. 2017 Feb:167:64-71. doi: 10.1016/j.jphotobiol.2016.12.012. Epub 2016 Dec 22.

Authors

Murugesan Dinesh¹, Selvaraj Mohana Roopan², Chinnadurai Immanuel Selvaraj³, Prabhakarn Arunachalam⁴

Affiliations

¹ Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632 014, Tamilnadu, India.
² Chemistry of Heterocycles & Natural Product Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632 014, Tamilnadu, India. Electronic address: mohanaroopan.s@vit.ac.in.
³ Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632 014, Tamilnadu, India. Electronic address: immanuelselvaraj@vit.ac.in.
⁴ Electrochemistry Research Group, Chemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

PMID: 28039791
DOI: 10.1016/j.jphotobiol.2016.12.012

Abstract

Ecofriendly synthesis of Palladium nanoparticles (PdNPs) were achieved using Phyllanthus emblica (P. emblica) seeds as reducing agent. Further the ecofriendly synthesized PdNPs were subjected for various analytical techniques like UV-Vis, FT-IR, XRD, Zeta potential, SEM and TEM. The results indicated that green synthesized PdNPs were spherical in shape with average particle size of 28±2nm with moderate stability. Further the synthesized PdNPs and extract were subjected for its antibacterial studies against various disease causing pathogens by agar well diffusion method. Seed extract resulted in 8.9±1.46mm against B. subtilis and PdNPs showed 9.6±1.10mm against S. aureus and synthesized PdNPs and extract were tested for hemolytic which resulted in 20% and 10% respectively. Toxicity studies were done against Artemia salina (A. salina). The LC₅₀ value of green synthesized P. emblica capped PdNPs and the P. emblica seed extract were found to be less toxic for A. salina with a value of 1.00μg/mL and 1.25μg/mL. In addition samples were checked for in vitro cytotoxicity assays on HeLa cell lines.

Keywords: Artemia salina; Haemolysis; HeLa cell lines; PdNPs; Phyllanthus emblica.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Cell Survival / drug effects*
Hemolysis / drug effects*
Humans
Metal Nanoparticles*
Microbial Sensitivity Tests
Microscopy, Electron / methods
Palladium / chemistry
Palladium / pharmacology*
Phyllanthus emblica / chemistry*
Plant Extracts / pharmacology*
Seeds / chemistry*
Spectrum Analysis
X-Ray Diffraction

Substances

Anti-Bacterial Agents
Plant Extracts
Palladium