Inhibitory effects of sodium azide on microbial growth in experimental resuspension of marine sediment

Léa Cabrol; Marianne Quéméneur; Benjamin Misson

doi:10.1016/j.mimet.2016.12.021

Inhibitory effects of sodium azide on microbial growth in experimental resuspension of marine sediment

J Microbiol Methods. 2017 Feb:133:62-65. doi: 10.1016/j.mimet.2016.12.021. Epub 2016 Dec 27.

Authors

Léa Cabrol¹, Marianne Quéméneur², Benjamin Misson³

Affiliations

¹ Aix Marseille Univ, Univ Toulon, CNRS, IRD, MIO UM 110, Mediterranean Institute of Oceanography, Marseille, France. Electronic address: lea.cabrol@mio.osupytheas.fr.
² Aix Marseille Univ, Univ Toulon, CNRS, IRD, MIO UM 110, Mediterranean Institute of Oceanography, Marseille, France.
³ PROTEE, EA 3819, Toulon University, CS 60584-83041 Toulon, Cedex 9, France.

PMID: 28039035
DOI: 10.1016/j.mimet.2016.12.021

Abstract

Sodium azide (NaN₃) was evaluated as inhibitor of microbial growth and activity in marine sediment resuspensions by monitoring the abundance of free-living and sessile bacteria using both flow cytometry and qPCR methods. Results show that 50mM of NaN₃ strongly inhibits bacterial growth under natural and enriched resource conditions.

Keywords: Inhibition; Marine sediment; Microbial growth; Sodium azide (NaN(3)); Sterilization; qPCR/cytometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / drug effects*
Bacteria / growth & development
Culture Media / chemistry*
DNA, Bacterial / isolation & purification
Dose-Response Relationship, Drug
Flow Cytometry
Geologic Sediments / microbiology*
Plankton / drug effects
Plankton / growth & development
Polymerase Chain Reaction
Sodium Azide / toxicity*

Substances

Culture Media
DNA, Bacterial
Sodium Azide