Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract

Govindasamy Rajakumar; Thandapani Gomathi; Muthu Thiruvengadam; V Devi Rajeswari; V N Kalpana; Ill-Min Chung

doi:10.1016/j.micpath.2016.12.019

Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract

Microb Pathog. 2017 Feb:103:123-128. doi: 10.1016/j.micpath.2016.12.019. Epub 2016 Dec 23.

Authors

Govindasamy Rajakumar¹, Thandapani Gomathi², Muthu Thiruvengadam¹, V Devi Rajeswari³, V N Kalpana³, Ill-Min Chung⁴

Affiliations

¹ Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University, Seoul 143 701, South Korea.
² Department of Chemistry, D.K.M. College for Women, Vellore, Tamil Nadu, India. Electronic address: drgoms1@gmail.com.
³ Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore -14, Tamil Nadu, India.
⁴ Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University, Seoul 143 701, South Korea. Electronic address: imchung@konkuk.ac.kr.

PMID: 28025099
DOI: 10.1016/j.micpath.2016.12.019

Abstract

The aim of this study is to develop an easy and eco-friendly method for the synthesis of Ag-NPs using extracts from the medicinal plant, Millettia pinnata flower extract and investigate the effects of Ag-NPs on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), antibacterial and cytotoxicity activity. UV-Vis peak at 438 nm confirmed the Ag-NPs absorbance. The SEM analysis results confirmed the presence of spherical shaped Ag-NPs by a huge disparity in the particle size distribution with an average size of 49 ± 0.9 nm. TEM images revealed the formation of Ag-NPs with spherical shape and sizes in the range between 16 and 38 nm. The Ag-NPs showed an excellent inhibitory efficacy against AChE and BChE. The highest antibacterial activity was found against Escherichia coli (20.25 ± 0.91 mm). These nanoparticles showed the cytotoxic effects against brine shrimp (artemia saliana) nauplii with a LD₅₀ value of 33.92.

Keywords: Anti-cholinesterase; Antibacteria; Electron microscopy; Millettia pinnata; Silver nanoparticles.

MeSH terms

Animals
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology*
Cholinesterase Inhibitors / chemistry
Cholinesterase Inhibitors / pharmacology*
Enzyme Activation / drug effects
Flowers / chemistry*
Green Chemistry Technology
Metal Nanoparticles* / administration & dosage
Metal Nanoparticles* / chemistry
Metal Nanoparticles* / ultrastructure
Microbial Sensitivity Tests
Millettia / chemistry*
Photoelectron Spectroscopy
Plant Extracts / chemistry
Plant Extracts / pharmacology*
Silver* / chemistry
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction

Substances

Anti-Bacterial Agents
Cholinesterase Inhibitors
Plant Extracts
Silver