Abstract
Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.
Keywords:
Escherichia coli, arabinose; Flp recombinase; Gene expression; Repression.
Copyright © 2016 Elsevier B.V. All rights reserved.
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Binding Sites
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Cloning, Molecular
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DNA Helicases / genetics
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DNA Helicases / metabolism
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DNA Nucleotidyltransferases / genetics*
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DNA Nucleotidyltransferases / metabolism
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Escherichia coli / enzymology*
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Escherichia coli / genetics*
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / metabolism
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Plasmids
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Promoter Regions, Genetic
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Recombination, Genetic
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Trans-Activators / genetics
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Trans-Activators / metabolism
Substances
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Bacterial Proteins
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Trans-Activators
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replication initiator protein
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Phosphotransferases (Alcohol Group Acceptor)
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ribulokinase
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DNA Nucleotidyltransferases
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FLP recombinase
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Site-specific recombinase
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DNA Helicases