Stringent control of FLP recombinase in Escherichia coli

J Microbiol Methods. 2017 Feb:133:52-54. doi: 10.1016/j.mimet.2016.12.018. Epub 2016 Dec 24.

Abstract

Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.

Keywords: Escherichia coli, arabinose; Flp recombinase; Gene expression; Repression.

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Cloning, Molecular
  • DNA Helicases / genetics
  • DNA Helicases / metabolism
  • DNA Nucleotidyltransferases / genetics*
  • DNA Nucleotidyltransferases / metabolism
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics*
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Plasmids
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Trans-Activators / genetics
  • Trans-Activators / metabolism

Substances

  • Bacterial Proteins
  • Trans-Activators
  • replication initiator protein
  • Phosphotransferases (Alcohol Group Acceptor)
  • ribulokinase
  • DNA Nucleotidyltransferases
  • FLP recombinase
  • Site-specific recombinase
  • DNA Helicases