Low-temperature thermal reduction of suspended graphene oxide film for electrical sensing of DNA-hybridization

Tun Wang; Hong-Chen Guo; Xin-Yi Chen; Miao Lu

doi:10.1016/j.msec.2016.11.026

Low-temperature thermal reduction of suspended graphene oxide film for electrical sensing of DNA-hybridization

Mater Sci Eng C Mater Biol Appl. 2017 Mar 1:72:62-68. doi: 10.1016/j.msec.2016.11.026. Epub 2016 Nov 9.

Authors

Tun Wang¹, Hong-Chen Guo¹, Xin-Yi Chen², Miao Lu³

Affiliations

¹ Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, 361005, PR China.
² Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, 361005, PR China. Electronic address: chenxinyi@xmu.edu.cn.
³ Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, 361005, PR China. Electronic address: lm@xmu.edu.cn.

PMID: 28024629
DOI: 10.1016/j.msec.2016.11.026

Abstract

A reduced graphene oxide (RGO) based capacitive real time bio-sensor was presented. Suspended graphene oxide (GO) film was assembled electrophoretically between the source and drain electrodes of a transistor and then reduced by annealing in hydrogen/nitrogen forming gas to optimize the surface functional groups and conductivity. The resonance frequency of the transmission coefficient (S₂₁) of the transistor was observed to shift with deoxyribonucleic acid (DNA)-hybridization, with a detecting limit of ~5nM. The advantages of the bio-sensing approach include low-noise frequency output, solution based real time detection and capable of on-chip integration.

Keywords: DNA detection; Suspended graphene oxide film; Thermal reduction.

MeSH terms

Biosensing Techniques
Cold Temperature
DNA / analysis*
Electrochemical Techniques* / instrumentation
Electrodes
Gases / chemistry
Graphite / chemistry*
Immobilized Nucleic Acids / chemistry
Limit of Detection
Microscopy, Atomic Force
Nucleic Acid Hybridization
Oxidation-Reduction
Oxides / chemistry

Substances

Gases
Immobilized Nucleic Acids
Oxides
Graphite
DNA