Assay of superoxide dismutase activity in a plate assay using WST-1

Alexander V Peskin; Christine C Winterbourn

doi:10.1016/j.freeradbiomed.2016.12.033

Assay of superoxide dismutase activity in a plate assay using WST-1

Free Radic Biol Med. 2017 Feb:103:188-191. doi: 10.1016/j.freeradbiomed.2016.12.033. Epub 2016 Dec 23.

Authors

Alexander V Peskin¹, Christine C Winterbourn²

Affiliations

¹ Centre for Free Radical Research, Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand.
² Centre for Free Radical Research, Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand. Electronic address: christine.winterbourn@otago.ac.nz.

PMID: 28017897
DOI: 10.1016/j.freeradbiomed.2016.12.033

Abstract

One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1. For reliable results with WST-1, certain aspects of the procedure need to be adhered to. This article describes an appropriate protocol that minimizes sources of variability.

Keywords: Assay; Superoxide dismutase; Tetrazolium; WST-1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Enzyme Assays / instrumentation
Enzyme Assays / methods*
Kinetics
Oxidation-Reduction
Reproducibility of Results
Solutions
Superoxide Dismutase / chemistry*
Tetrazolium Salts / chemistry*

Substances

2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium
Solutions
Tetrazolium Salts
Superoxide Dismutase