Hemolytic Activity of Antimicrobial Peptides

Alberto Oddo; Paul R Hansen

doi:10.1007/978-1-4939-6737-7_31

Hemolytic Activity of Antimicrobial Peptides

Methods Mol Biol. 2017:1548:427-435. doi: 10.1007/978-1-4939-6737-7_31.

Authors

Alberto Oddo¹, Paul R Hansen²

Affiliations

¹ Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark.
² Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark. prh@sund.ku.dk.

PMID: 28013523
DOI: 10.1007/978-1-4939-6737-7_31

Abstract

For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human red blood cells, using melittin as positive control.

Keywords: Antimicrobial peptides; Hemolysis; Melittin; Red blood cells.

MeSH terms

Anti-Infective Agents / pharmacology*
Antimicrobial Cationic Peptides / pharmacology*
Dose-Response Relationship, Drug
Erythrocytes / drug effects
Hemolysis / drug effects
Humans
Melitten / pharmacology

Substances

Anti-Infective Agents
Antimicrobial Cationic Peptides
Melitten