Chemical Synthesis of Antimicrobial Peptides

Lena Münzker; Alberto Oddo; Paul R Hansen

doi:10.1007/978-1-4939-6737-7_3

Chemical Synthesis of Antimicrobial Peptides

Methods Mol Biol. 2017:1548:35-49. doi: 10.1007/978-1-4939-6737-7_3.

Authors

Lena Münzker¹, Alberto Oddo¹, Paul R Hansen²

Affiliations

¹ Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, Copenhagen, 2100, Denmark.
² Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, Copenhagen, 2100, Denmark. prh@sund.ku.dk.

PMID: 28013495
DOI: 10.1007/978-1-4939-6737-7_3

Abstract

Solid-phase peptide synthesis (SPPS) is the method of choice for chemical synthesis of peptides. In this nonspecialist review, we describe commonly used resins, linkers, protecting groups, and coupling reagents in 9-fluorenylmethyloxycarbonyl (Fmoc) SPPS. Finally, a detailed protocol for manual Fmoc SPPS is presented.

Keywords: 9-Fluorenylmethyloxycarbonyl (Fmoc) solid-phase peptide synthesis; DIC; Oxyma.

MeSH terms

Amino Acids / chemistry
Anti-Infective Agents / chemical synthesis*
Anti-Infective Agents / chemistry
Anti-Infective Agents / pharmacology
Molecular Structure
Peptides / chemical synthesis*
Peptides / chemistry
Peptides / pharmacology
Solid-Phase Synthesis Techniques* / instrumentation
Solid-Phase Synthesis Techniques* / methods

Substances

Amino Acids
Anti-Infective Agents
Peptides