High efficiency of inorganic nitrogen removal by integrating biofilm-electrode with constructed wetland: Autotrophic denitrifying bacteria analysis

Junfeng Wang; Yuhui Wang; Junhong Bai; Zhaowei Liu; Xinshan Song; Dengming Yan; Asaminew Abiyu; Zhimiao Zhao; Denghua Yan

doi:10.1016/j.biortech.2016.12.046

High efficiency of inorganic nitrogen removal by integrating biofilm-electrode with constructed wetland: Autotrophic denitrifying bacteria analysis

Bioresour Technol. 2017 Mar:227:7-14. doi: 10.1016/j.biortech.2016.12.046. Epub 2016 Dec 16.

Authors

Junfeng Wang¹, Yuhui Wang², Junhong Bai³, Zhaowei Liu⁴, Xinshan Song¹, Dengming Yan¹, Asaminew Abiyu¹, Zhimiao Zhao¹, Denghua Yan⁵

Affiliations

¹ College of Environmental Science and Engineering, State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, Shanghai 201620, China.
² College of Environmental Science and Engineering, State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, Shanghai 201620, China. Electronic address: yhwang@dhu.edu.cn.
³ School of Environment, Beijing Normal University, Beijing 100038, China.
⁴ State Key Laboratory Hydroscience and Engineering, Tsinghua University, Beijing 100084, China.
⁵ China Institute of Water Resource and Hydropower Research, Beijing 100038, China.

PMID: 28012375
DOI: 10.1016/j.biortech.2016.12.046

Abstract

The constructed wetland coupled with biofilm-electrode reactor (CW-BER) is a novel technology to treat wastewater with a relatively high level of total inorganic nitrogen (TIN) concentration. The main objective of this study is to investigate the effects of C/Ns, TIN concentrations, current intensities, and pH on the removal of nitrogen in CW-BER; a control system (CW) was also constructed and operated with similar influent conditions. Results indicated that the current, inorganic carbon source and hydrogen generated by the micro-electric field could significantly improve the inorganic nitrogen removal with in CW-BER, and the enhancement of average removal rate on NH₃-N, NO₃-N, and TIN was approximately maintained at 5-28%, 5-26%, and 3-24%, respectively. The appropriate operation conditions were I=10mA and pH=7.5 in CW-BER. In addition, high-throughput sequencing analysis implied that the CW-BER reactor has been improved with the relative abundance of autotrophic denitrifying bacteria (Thiobacillus sp.).

Keywords: Autotrophic denitrifying bacteria; Biofilm-electrode reactor; Constructed wetland; Current intensity; Inorganic nitrogen removal.

MeSH terms

Autotrophic Processes
Bacteria
Biofilms*
Bioreactors / microbiology*
Denitrification*
Electrodes
Nitrogen / analysis
Nitrogen / chemistry
Wastewater / chemistry
Water Purification / methods*
Wetlands*

Substances

Waste Water
Nitrogen