Aerobic and anaerobic biosynthesis of nano-selenium for remediation of mercury contaminated soil

Abstract

Selenium (Se) nanoparticles are often synthesized by anaerobes. However, anaerobic bacteria cannot be directly applied for bioremediation of contaminated top soil which is generally aerobic. In this study, a selenite-reducing bacterium, Citrobacter freundii Y9, demonstrated high selenite reducing power and produced elemental nano-selenium nanoparticles (nano-Se⁰) under both aerobic and anaerobic conditions. The biogenic nano-Se⁰ converted 45.8-57.1% and 39.1-48.6% of elemental mercury (Hg⁰) in the contaminated soil to insoluble mercuric selenide (HgSe) under anaerobic and aerobic conditions, respectively. Addition of sodium dodecyl sulfonate enhanced Hg⁰ remediation, probably owing to the release of intracellular nano-Se⁰ from the bacterial cells for Hg fixation. The reaction product after remediation was identified as non-reactive HgSe that was formed by amalgamation of nano-Se⁰ and Hg⁰. Biosynthesis of nano-Se⁰ both aerobically and anaerobically therefore provides a versatile and cost-effective remediation approach for Hg⁰-contaminated surface and subsurface soils, where the redox potential often changes dramatically.

Keywords: Bioremediation; Mercury; Metal immobilization; Selenium; Selenium nanoparticles.