Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type

K S Im; J H Kim; A J Graef; I Cornax; D M Seelig; M G O'Sullivan; R C Kovi; J F Modiano

doi:10.1016/j.jcpa.2016.11.271

Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type

J Comp Pathol. 2017 Jan;156(1):37-41. doi: 10.1016/j.jcpa.2016.11.271. Epub 2016 Dec 20.

Authors

K S Im¹, J H Kim², A J Graef², I Cornax³, D M Seelig², M G O'Sullivan³, R C Kovi³, J F Modiano⁴

Affiliations

¹ Animal Cancer Care and Research Program, University of Minnesota, St. Paul, USA; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, USA; Masonic Cancer Center, University of Minnesota, Minneapolis, USA; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA.
² Animal Cancer Care and Research Program, University of Minnesota, St. Paul, USA; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, USA; Masonic Cancer Center, University of Minnesota, Minneapolis, USA.
³ Animal Cancer Care and Research Program, University of Minnesota, St. Paul, USA; Masonic Cancer Center, University of Minnesota, Minneapolis, USA; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA.
⁴ Animal Cancer Care and Research Program, University of Minnesota, St. Paul, USA; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, USA; Masonic Cancer Center, University of Minnesota, Minneapolis, USA. Electronic address: modiano@umn.edu.

Abstract

The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.

Keywords: dog; enteropathy-associated T-cell lymphoma; immunodeficient mouse model; patient-derived tumour xenograft.

Publication types

Case Reports

MeSH terms

Animals
Disease Models, Animal*
Dog Diseases*
Dogs
Enteropathy-Associated T-Cell Lymphoma / veterinary*
Female
Heterografts
Male
Mice
Mice, Nude

Grants and funding

P30 CA077598/CA/NCI NIH HHS/United States