Dataset of proteins mapped on HepG2 cells and those differentially abundant after expression of the dengue non-structural 1 protein

Kíssila Rabelo; Monique R O Trugilho; Simone M Costa; André T S Ferreira; Paulo C Carvalho; Jonas Perales; Ada M B Alves

doi:10.1016/j.dib.2016.11.083

Dataset of proteins mapped on HepG2 cells and those differentially abundant after expression of the dengue non-structural 1 protein

Data Brief. 2016 Dec 6:10:248-263. doi: 10.1016/j.dib.2016.11.083. eCollection 2017 Feb.

Authors

Kíssila Rabelo¹, Monique R O Trugilho², Simone M Costa¹, André T S Ferreira², Paulo C Carvalho³, Jonas Perales², Ada M B Alves¹

Affiliations

¹ Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
² Laboratory of Toxinology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil.
³ Laboratory for Proteomics and Protein Engineering, Carlos Chagas Institute, Fiocruz, Paraná, Brazil.

Abstract

The data supplied in this article are related to the research article entitled "The effect of the dengue non-structural 1 protein expression over the HepG2 cell proteins in a proteomic approach" (K. Rabelo, M.R. Trugillo, S.M. Costa, B.A. Pereira, O.C. Moreira, A.T. Ferreira et al., 2016) [1]. The present article provides the inventory of peptides and proteins mapped in a hepatocyte cell line (HepG2) by mass spectrometry in the presence of the non-structural protein 1 (NS1) of Dengue 2 virus (DENV2). Cells were transfected with pcENS1 plasmid, which encodes the DENV2 NS1 protein, or the controls pcDNA3 (negative control) or pMAXGFP, encoding the green fluorescent protein (GFP), a protein unrelated to dengue. Differentially abundant protein lists were obtained by comparing cells transfected with pcENS1 and controls.

Keywords: DENV; HepG2 cells; Mass spectrometry; NS1.