Validation of the Microreader™ 23sp ID system: A new STR 23-plex system for forensic application

Forensic Sci Int Genet. 2017 Mar:27:67-73. doi: 10.1016/j.fsigen.2016.12.005. Epub 2016 Dec 12.

Abstract

Microreader™ 23sp ID system is a new 23-plex STR genotyping system that amplified 21 non-CODIS STR loci (D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290 and D5S2500), one CODIS STR locus (D16S539) and the amelogenin locus in one reaction. Microreader™ 23sp ID system was validated according to the guidelines of "Validation Guidelines for DNA Analysis Methods (2012)" described by the Scientific Working Group on DNA Analysis Methods (SWGDAM), including PCR-based studies, sensitivity study, precision and accuracy evaluation, stutter percentage and peak height ratio, inhibitors, species specificity and DNA mixture studies. Our results suggested that Microreader™ 23sp ID system is a useful tool for identification and parentage testing.

Keywords: Forensic science; Microreader™ 23sp ID system; Short tandem repeat (STR); Validation.

Publication types

  • Validation Study

MeSH terms

  • Amelogenin / genetics
  • Animals
  • Asian People / genetics
  • China
  • DNA Fingerprinting*
  • Ethnicity / genetics
  • Genotype
  • Humans
  • Microsatellite Repeats*
  • Multiplex Polymerase Chain Reaction / methods*
  • Species Specificity

Substances

  • Amelogenin