Characterization of xanthan gum produced from glycerol by a mutant strain Xanthomonas campestris CCTCC M2015714

Zichao Wang; Jianrong Wu; Li Zhu; Xiaobei Zhan

doi:10.1016/j.carbpol.2016.10.033

Characterization of xanthan gum produced from glycerol by a mutant strain Xanthomonas campestris CCTCC M2015714

Carbohydr Polym. 2017 Feb 10:157:521-526. doi: 10.1016/j.carbpol.2016.10.033. Epub 2016 Oct 13.

Authors

Zichao Wang¹, Jianrong Wu¹, Li Zhu², Xiaobei Zhan³

Affiliations

¹ The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
² Wuxi Galaxy Biotech Co. Ltd., Wuxi, Jiangsu, 214125, China.
³ The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: xbzhan@yahoo.com.

PMID: 27987957
DOI: 10.1016/j.carbpol.2016.10.033

Abstract

Xanthan gum was produced by a mutant strain X. campestris CCTCC M2015714 with glycerol as the sole carbon source. The monosaccharide composition and molar ratio of xanthan gum produced from glycerol are glucose: mannose: glucuronic acid=2.0:1.65:1.0. Meanwhile, chemical structure of xanthan gum produced from glycerol is similar to that of the commercial xanthan through FT-IR and NMR. Remarkably, the molecular weight of xanthan gum produced using our method (3.0±0.14×10⁶Da) is about half that of the commercial one (5.8±0.25×10⁶Da), and the consistency index (K) of which is less than 1/10 that of the commercial xanthan. This work paves the way for xanthan production from glycerol and is useful for studying the structure/application of xanthan gum.

Keywords: Biopolymer; Characterization; Glycerol; Xanthan gum.

MeSH terms

Glycerol / chemistry*
Polysaccharides, Bacterial / chemistry*
Xanthomonas campestris / metabolism*

Substances

Polysaccharides, Bacterial
Glycerol
xanthan gum