Fast Screening of Tissue Samples for Glycogen

J Pharm Biomed Anal. 2017 Feb 20:135:16-19. doi: 10.1016/j.jpba.2016.12.009. Epub 2016 Dec 8.

Abstract

Screening of tissue samples for glycogen is very important in assessing the ageing, but also the state of health of the tissue. Therefore, two needle stochastic sensors based on maltodextrins presenting different dextrose equivalence (DE) MD-m (DE 13.0-17.0), and MD-M (DE 16.5-19.5) immobilized in diamond paste (obtained from synthetic diamond and paraffin oil) were designed and characterized. These stochastic sensors were used reliable for both qualitative and quantitative analysis for the assay of glycogen in tissue samples with limits of determination as low as 1fmol L-1.

Keywords: Glycogen; Needle stochastic sensor; Screening system; Tissue sample.

MeSH terms

  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Colon / chemistry*
  • Diamond / chemistry
  • Electrodes
  • Glycogen / analysis*
  • Humans
  • Oils / chemistry
  • Paraffin / chemistry
  • Polysaccharides / chemistry
  • Skin / chemistry*
  • Time Factors

Substances

  • Oils
  • Polysaccharides
  • Diamond
  • maltodextrin
  • Paraffin
  • paraffin oils
  • Glycogen