A methodology for the determination of tomato phenolic acids and flavonoids has been developed combining MEKC and DAD detection. The influence on polyphenol separation of pH and background electrolyte, BGE (borax, acetonitrile, methanol and SDS concentrations), was studied and optimized using response surface methodology and weighted desirability function. Separation of polyphenols was achieved within 20min at 15°C using 11.3mM borax and 11.2mM SDS adjusted to pH 8.5 as BGE. Validation was performed using standards and tomato extracts. Recoveries ranged from 77 to 106%. Acceptable repeatabilities were obtained for peak area (%RSD <3.1% and <3.7%) and migration times (%RSD <0.2% and <1.4%) for intra- and inter-day respectively. Detection limits ranged between 0.8 and 3.8mgkg-1. Five and seven of these polyphenols were determined in samples of tomato and related species. This methodology will be valuable tool in breeding programs, analyzing a large number of samples.
Keywords: Caffeic acid (PubChem CID: 689043); Chlorogenic acid (PubChem CID: 1794427); Functional quality; Kaempferol (PubChem CID: 5280863); MEKC; Myricetin (PubChem CID: 5281672); Naringenin (PubChem CID: 932); Plant breeding; Polyphenol; Quercetin (PubChem CID: 5280343); Response surface methodology; Rutin (PubChem CID: 5280805); p-Coumaric acid (PubChem CID: 637542); trans-Ferulic acid (PubChem CID: 445858).
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