Extraction of bioactives from Orthosiphon stamineus using microwave and ultrasound-assisted techniques: Process optimization and scale up

Chung-Hung Chan; Tiam-You See; Rozita Yusoff; Gek-Cheng Ngoh; Kien-Woh Kow

doi:10.1016/j.foodchem.2016.11.016

Extraction of bioactives from Orthosiphon stamineus using microwave and ultrasound-assisted techniques: Process optimization and scale up

Food Chem. 2017 Apr 15:221:1382-1387. doi: 10.1016/j.foodchem.2016.11.016. Epub 2016 Nov 4.

Authors

Chung-Hung Chan¹, Tiam-You See², Rozita Yusoff³, Gek-Cheng Ngoh², Kien-Woh Kow⁴

Affiliations

¹ Advanced Oleochemical Technology Division, Malaysian Palm Oil Board, 43000 Kajang, Selangor, Malaysia. Electronic address: chan@mpob.gov.my.
² Centre for Separation Science and Technology (CSST), Department of Chemical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
³ Centre for Separation Science and Technology (CSST), Department of Chemical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: ryusoff@um.edu.my.
⁴ Department of Chemical and Petroleum Engineering, Faculty of Engineering, Technology & Built Environment, UCSI University, Kuala Lumpur 56000, Malaysia.

PMID: 27979103
DOI: 10.1016/j.foodchem.2016.11.016

Abstract

This work demonstrated the optimization and scale up of microwave-assisted extraction (MAE) and ultrasonic-assisted extraction (UAE) of bioactive compounds from Orthosiphon stamineus using energy-based parameters such as absorbed power density and absorbed energy density (APD-AED) and response surface methodology (RSM). The intensive optimum conditions of MAE obtained at 80% EtOH, 50mL/g, APD of 0.35W/mL, AED of 250J/mL can be used to determine the optimum conditions of the scale-dependent parameters i.e. microwave power and treatment time at various extraction scales (100-300mL solvent loading). The yields of the up scaled conditions were consistent with less than 8% discrepancy and they were about 91-98% of the Soxhlet extraction yield. By adapting APD-AED method in the case of UAE, the intensive optimum conditions of the extraction, i.e. 70% EtOH, 30mL/g, APD of 0.22W/mL, AED of 450J/mL are able to achieve similar scale up results.

Keywords: Bioactive compounds; Microwave extraction; Orthosiphon stamineus; Scale up; Ultrasonic extraction.

MeSH terms

Microwaves / therapeutic use*
Orthosiphon / metabolism*
Ultrasonics / methods*