Pin1 Knockout Mice: A Model for the Study of Tau Pathology in Alzheimer's Disease

Methods Mol Biol. 2017:1523:415-425. doi: 10.1007/978-1-4939-6598-4_28.

Abstract

Pin1 knockout in mice causes age-dependent neuropathy characterized by motor and behavioral deficits, tau hyper phosphorylation, tau filament formation, and neuronal degradation. Here, we describe the methods with mouse behavior test, immunostaining, and immunoblotting to detect many aspects of neurodegeneration in Pin1 knockout mice.

Keywords: Alzheimer’s disease; Behavior test; Cis P-tau; Immunoblotting; Immunohistochemistry; Pin1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Alzheimer Disease / genetics
  • Alzheimer Disease / metabolism
  • Alzheimer Disease / pathology
  • Animals
  • Behavior Rating Scale
  • Disease Models, Animal
  • Immunohistochemistry
  • Mice
  • Mice, Knockout
  • NIMA-Interacting Peptidylprolyl Isomerase / deficiency
  • NIMA-Interacting Peptidylprolyl Isomerase / genetics*
  • Tauopathies / genetics*
  • Tauopathies / metabolism*
  • Tauopathies / pathology
  • tau Proteins / genetics
  • tau Proteins / metabolism*

Substances

  • NIMA-Interacting Peptidylprolyl Isomerase
  • tau Proteins