Single molecule based SNP detection using designed DNA carriers and solid-state nanopores

Chem Commun (Camb). 2016 Dec 22;53(2):436-439. doi: 10.1039/c6cc08621g.

Abstract

Single nucleotide polymorphisms (SNPs) play a crucial role as molecular markers in medical and diagnostic application. We demonstrate a nanopore based method for SNP detection at the single molecule level. Designed DNA carriers are used to distinguish DNA strands containing only one single base difference and follow strand displacement kinetics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • DNA / chemistry
  • DNA / genetics*
  • DNA Probes / chemistry
  • Kinetics
  • Models, Molecular
  • Nanopores*
  • Nanotechnology
  • Nucleic Acid Conformation
  • Polymorphism, Single Nucleotide*

Substances

  • DNA Probes
  • DNA