Plant architecture and grain yield are regulated by the novel DHHC-type zinc finger protein genes in rice (Oryza sativa L.)

Bo Zhou; Jian Zhong Lin; Dan Peng; Yuan Zhu Yang; Ming Guo; Dong Ying Tang; Xiaofeng Tan; Xuan Ming Liu

doi:10.1016/j.plantsci.2016.08.015

Plant architecture and grain yield are regulated by the novel DHHC-type zinc finger protein genes in rice (Oryza sativa L.)

Plant Sci. 2017 Jan:254:12-21. doi: 10.1016/j.plantsci.2016.08.015. Epub 2016 Aug 31.

Authors

Bo Zhou¹, Jian Zhong Lin², Dan Peng³, Yuan Zhu Yang⁴, Ming Guo², Dong Ying Tang², Xiaofeng Tan⁵, Xuan Ming Liu⁶

Affiliations

¹ College of Bioscience and Biotechnology of Central South University of Forestry and Technology, Changsha 410018, Hunan, China; Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, 410018 Changsha, China.
² Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Changsha 410082, Hunan, China; College of Biology, Hunan University, Changsha 410082, Hunan, China; Bioenergy and Biomaterial Research Center, College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, Hunan, China.
³ College of Bioscience and Biotechnology of Central South University of Forestry and Technology, Changsha 410018, Hunan, China; Academy of Seed Industry of Hunan Yahua, Changsha 410013, Hunan, China; Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, 410018 Changsha, China.
⁴ Academy of Seed Industry of Hunan Yahua, Changsha 410013, Hunan, China.
⁵ Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, 410018 Changsha, China.
⁶ Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, Changsha 410082, Hunan, China; College of Biology, Hunan University, Changsha 410082, Hunan, China; Bioenergy and Biomaterial Research Center, College of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, Hunan, China. Electronic address: xmL05@hnu.edu.cn.

PMID: 27964781
DOI: 10.1016/j.plantsci.2016.08.015

Abstract

In many plants, architecture and grain yield are affected by both the environment and genetics. In rice, the tiller is a vital factor impacting plant architecture and regulated by many genes. In this study, we cloned a novel DHHC-type zinc finger protein gene Os02g0819100 and its alternative splice variant OsDHHC1 from the cDNA of rice (Oryza sativa L.), which regulate plant architecture by altering the tiller in rice. The tillers increased by about 40% when this type of DHHC-type zinc finger protein gene was over-expressed in Zhong Hua 11 (ZH11) rice plants. Moreover, the grain yield of transgenic rice increased approximately by 10% compared with wild-type ZH11. These findings provide an important genetic engineering approach for increasing rice yields.

Keywords: Alternative splicing; DHHC-type zinc finger protein; OsDHHC1; Plant architecture; Rice.

MeSH terms

Cloning, Molecular
Genetic Engineering
Oryza / anatomy & histology
Oryza / genetics
Oryza / growth & development*
Plant Proteins / genetics
Plant Proteins / metabolism
Plant Proteins / physiology*
Plants, Genetically Modified / growth & development
Protein Isoforms / chemistry
Sequence Analysis, Protein
Zinc Fingers

Substances

Plant Proteins
Protein Isoforms