Development of real-time immuno-PCR for the quantitative detection of mycobacterial PstS1 in tuberculosis patients

J Microbiol Methods. 2017 Jan:132:134-138. doi: 10.1016/j.mimet.2016.12.006. Epub 2016 Dec 7.

Abstract

A novel indirect real-time immuno-polymerase chain reaction (RT-I-PCR) assay, an evolution of I-PCR, was developed for the quantitative detection of Mycobacterium tuberculosis PstS1 (Rv0934) with a wide dynamic range of 10ng/mL to 1pg/mL in body fluids of tuberculosis (TB) patients, which may monitor the dynamics of disease.

Keywords: Diagnosis; Mycobacterium tuberculosis; PstS1; Real-time immuno-PCR; Tuberculosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • Adolescent
  • Adult
  • Bacterial Proteins / genetics*
  • Bacterial Typing Techniques
  • Case-Control Studies
  • Colony Count, Microbial
  • DNA, Bacterial / isolation & purification
  • Evaluation Studies as Topic
  • Female
  • Humans
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis / isolation & purification*
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Tuberculosis / diagnosis*
  • Tuberculosis / microbiology
  • Young Adult

Substances

  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • DNA, Bacterial
  • PstS-1 protein, Mycobacterium tuberculosis