Draft genome of a South African strain of Pectobacterium carotovorum subsp. brasiliense

Khayalethu Ntushelo; Joseph Mafofo

doi:10.1016/j.bjm.2016.06.016

Draft genome of a South African strain of Pectobacterium carotovorum subsp. brasiliense

Braz J Microbiol. 2017 Jan-Mar;48(1):11-12. doi: 10.1016/j.bjm.2016.06.016. Epub 2016 Nov 30.

Authors

Khayalethu Ntushelo¹, Joseph Mafofo²

Affiliations

¹ University of South Africa, Science Campus, Department of Agriculture and Animal Health, Florida, South Africa. Electronic address: ntushk@unisa.ac.za.
² Formerly, Agricultural Research Council - Biotechnology Platform, Onderstepoort, South Africa.

Abstract

The draft genome of Pectobacterium carotovorum subsp. brasiliense (Pcb) which causes blackleg of potato was submitted to the NCBI and released with reference number NZ_LGRF00000000.1. The estimated genome size based on the draft genome assembly is 4,820,279bp from 33 contigs ranging in length from 444 to 1,660,019 nucleotides. The genome annotation showed 4250 putative genes, 4114 CDS and 43 pseudo-genes. Three complete rRNA gene species were detected: nine 5S, one 16S and one 23S. Other partial rRNA gene fragments were also identified, nine 16S rRNA and three 23S rRNA. A total of 69 tRNA genes and one ncRNA gene were also annotated in this genome.

Keywords: Genome sequencing; Illumina; Pectobacterium carotovorum subsp. brasiliensis.

MeSH terms

Computational Biology / methods
Genome, Bacterial*
Genomics* / methods
High-Throughput Nucleotide Sequencing
Molecular Sequence Annotation
Pectobacterium carotovorum / genetics*
Sequence Analysis, DNA