Abstract
Gastrodia elata (GE), which belongs to the Orchidaceae family, was found to possess anti-inflammatory activity. However, the effect of GE on inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) remains largely unknown. Thus, the aim of this study was to investigate the effects of GE on tumor necrosis factor-α (TNF-α)-induced inflammatory response in RA-FLS and the underlying molecular mechanism was also explored. Our results demonstrated that GE significantly attenuated TNF-α-induced IL-6 and IL-8 production in RA-FLS. GE also inhibited TNF-α-induced MMP-3 and MMP-13 expression in RA-FLS. Furthermore, pretreatment with GE significantly attenuated TNF-α-induced the expression of p-p65 and IκBα degradation in RA-FLS. In conclusion, this study demonstrated for the first time that GE attenuated inflammatory response by inhibiting the NF-κB pathway signaling in RA-FLS. Thus, GE might have a therapeutic potential towards the treatment of RA.
Keywords:
Gastrodia elata; Inflammation; Rheumatoid arthritis fibroblast-like synoviocytes; Tumor necrosis factor-α.
Copyright © 2016. Published by Elsevier Masson SAS.
MeSH terms
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Antirheumatic Agents / isolation & purification
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Antirheumatic Agents / pharmacology*
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Arthritis, Rheumatoid / drug therapy*
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Arthritis, Rheumatoid / genetics
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Arthritis, Rheumatoid / metabolism
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Arthritis, Rheumatoid / pathology
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Dose-Response Relationship, Drug
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Fibroblasts / drug effects*
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Fibroblasts / metabolism
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Fibroblasts / pathology
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Gastrodia* / chemistry
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Humans
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Inflammation Mediators / metabolism*
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Interleukin-6 / metabolism
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Interleukin-8 / metabolism
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Matrix Metalloproteinase 13 / genetics
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Matrix Metalloproteinase 13 / metabolism
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Matrix Metalloproteinase 3 / genetics
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Matrix Metalloproteinase 3 / metabolism
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NF-KappaB Inhibitor alpha / metabolism
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NF-kappa B / metabolism*
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Phosphorylation
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Phytotherapy
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plants, Medicinal
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Proteolysis
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Signal Transduction / drug effects
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Synovial Membrane / drug effects*
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Synovial Membrane / metabolism
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Synovial Membrane / pathology
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Time Factors
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Transcription Factor RelA / metabolism
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Anti-Inflammatory Agents
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Antirheumatic Agents
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CXCL8 protein, human
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IL6 protein, human
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Inflammation Mediators
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Interleukin-6
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Interleukin-8
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NF-kappa B
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Plant Extracts
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RELA protein, human
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Transcription Factor RelA
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Tumor Necrosis Factor-alpha
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NF-KappaB Inhibitor alpha
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MMP13 protein, human
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Matrix Metalloproteinase 13
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MMP3 protein, human
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Matrix Metalloproteinase 3