High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform

Sci Rep. 2016 Dec 9:6:38856. doi: 10.1038/srep38856.

Abstract

With great advances in the field of in vitro brain modelling, the challenge is now to implement these technologies for development and evaluation of new drug candidates. Here we demonstrate a method for culturing three-dimensional networks of spontaneously active neurons and supporting glial cells in a microfluidic platform. The high-throughput nature of the platform in combination with its compatibility with all standard laboratory equipment allows for parallel evaluation of compound effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials
  • Cell Culture Techniques / instrumentation*
  • Cell Shape
  • Coculture Techniques
  • Collagen
  • Drug Combinations
  • High-Throughput Screening Assays / methods*
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Lab-On-A-Chip Devices*
  • Laminin
  • Neural Stem Cells / cytology*
  • Neurites / ultrastructure
  • Neurogenesis
  • Neuroglia / cytology
  • Neuroglia / drug effects*
  • Neurons / cytology
  • Neurons / drug effects*
  • Neurotoxins / pharmacology
  • Organoids / drug effects*
  • Proteoglycans

Substances

  • Drug Combinations
  • Laminin
  • Neurotoxins
  • Proteoglycans
  • matrigel
  • Collagen