Measurement of Metabolome Samples Using Liquid Chromatography-Mass Spectrometry, Data Acquisition, and Processing

Tomáš Pluskal; Mitsuhiro Yanagida

doi:10.1101/pdb.prot091561

Measurement of Metabolome Samples Using Liquid Chromatography-Mass Spectrometry, Data Acquisition, and Processing

Cold Spring Harb Protoc. 2016 Dec 1;2016(12). doi: 10.1101/pdb.prot091561.

Authors

Tomáš Pluskal¹, Mitsuhiro Yanagida¹

Affiliation

¹ G0 Cell Unit, Okinawa Institute of Science and Technology Graduate University (OIST), Onna-son, Kunigami, Okinawa 904-0495, Japan.

PMID: 27934688
DOI: 10.1101/pdb.prot091561

Abstract

We present a protocol for metabolomic sample measurement using hydrophilic interaction chromatography (HILIC) combined with high-resolution Orbitrap mass spectrometry (MS). We also introduce a raw data processing method using MZmine 2 software, and include a list of 111 metabolite peaks (with their m/z values and retention times) previously identified in metabolome samples using this method.

MeSH terms

Chromatography, Liquid / methods*
Mass Spectrometry / methods*
Metabolome*
Metabolomics / methods*
Schizosaccharomyces / chemistry*