Human embryonic stem cells derived from abnormal blastocyst donated by polycystic kidney syndrome patient

Qi Ouyang; Xiaoying Zhou; Jing Chen; Juan Du; Guangxiu Lu; Ge Lin; Yi Sun

doi:10.1016/j.scr.2016.11.001

Human embryonic stem cells derived from abnormal blastocyst donated by polycystic kidney syndrome patient

Stem Cell Res. 2016 Nov;17(3):637-639. doi: 10.1016/j.scr.2016.11.001. Epub 2016 Nov 5.

Authors

Qi Ouyang¹, Xiaoying Zhou², Jing Chen³, Juan Du⁴, Guangxiu Lu⁵, Ge Lin⁶, Yi Sun⁷

Affiliations

¹ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China.
² National Engineering and Research Center of Human Stem Cell, Changsha, China.
³ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
⁴ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁵ National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁶ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁷ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China. Electronic address: sunyi66@csu.edu.cn.

PMID: 27934598
DOI: 10.1016/j.scr.2016.11.001

Abstract

Human embryonic stem cell (hESC) line chHES-468 was derived from abnormal blastocyst donated by polycystic kidney syndrome (PKD) patient after preimplantation genetic diagnosis (PGD) treatment. DNA sequencing analysis confirmed that chHES-468 cell line carried a heterozygous mutation, c.10526_10527delAG, of PKD1. Characteristic tests proved that the chHES-468 cell line presented typical markers of pluripotency and had the capability to form the three germ layers both in vitro and in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Blastocyst / cytology
Cell Differentiation
Cell Line
Cellular Reprogramming
Embryoid Bodies / cytology
Embryoid Bodies / metabolism
Female
Gene Deletion
Heterozygote
Human Embryonic Stem Cells / cytology*
Human Embryonic Stem Cells / metabolism
Human Embryonic Stem Cells / transplantation
Humans
Karyotype
Male
Mice
Mice, SCID
Plasmids / genetics
Plasmids / metabolism
Polycystic Kidney Diseases / genetics
Polycystic Kidney Diseases / pathology*
TRPP Cation Channels / genetics*
Teratoma / pathology
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

TRPP Cation Channels
Transcription Factors
polycystic kidney disease 1 protein