Inkjetting Plus Peroxidase-Mediated Hydrogelation Produces Cell-Laden, Cell-Sized Particles with Suitable Characters for Individual Applications

Abstract

The authors report a method to prepare cell-laden, cell-sized microparticles from various materials suitable for individual applications. The method includes a piezoelectric inkjetting technology and a horseradish peroxidase (HRP)-catalyzed crosslinking reaction. The piezoelectric inkjetting technology enables production of cell-laden, cell-sized (20-60 μm) droplets from a polymer aqueous solution. The HRP-catalyzed crosslinking of the polymer in the ejected solution enables production of spherical microparticles from various materials. Superior cytocompatibility of the microencapsulation method is confirmed from the viability and growth profiles of normal murine mammary gland epithelial cells.

Keywords: cell-laden microparticles; horseradish peroxidase; hydrogels; inkjetting; microencapsulation.