Programmed Assembly of Host-Guest Protein Crystals

Thaddaus R Huber; Luke F Hartje; Eli C McPherson; Ann E Kowalski; Christopher D Snow

doi:10.1002/smll.201602703

Programmed Assembly of Host-Guest Protein Crystals

Small. 2017 Feb;13(7). doi: 10.1002/smll.201602703. Epub 2016 Dec 7.

Authors

Thaddaus R Huber¹, Luke F Hartje¹, Eli C McPherson¹, Ann E Kowalski¹, Christopher D Snow¹

Affiliation

¹ Chemical and Biological Engineering, Colorado State University, 1301 Campus Delivery, Fort Collins, Colorado, 80523, USA.

PMID: 27925463
DOI: 10.1002/smll.201602703

Abstract

The binding and release of guest fluorescent proteins inside a protein crystal with 13 nm axial pores is controlled. Spatially segregated guest protein loading is achieved via sequential binding and release stages. Additionally, selective stabilization of the crystal exterior results in hollow crystalline shells.

Keywords: host-guest protein crystals; metal affinity capture; nanoporous materials; protein engineering.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacterial Proteins / chemistry*
Campylobacter jejuni / metabolism
Crystallization
Fluorescent Dyes / chemistry
Time Factors

Substances

Bacterial Proteins
Fluorescent Dyes