Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions

Methods Mol Biol. 2017:1487:163-174. doi: 10.1007/978-1-4939-6424-6_12.

Abstract

Caveolin1, the main component of caveolae, plays a major role in regulating cell motility, gene expression, and cytoskeleton remodeling downstream of many membrane receptors. Here, we summarize different techniques set up to study changes in cell morphology and cell motility regulated by ERK/caveolin1 interactions during induction of epithelial mesenchymal transition (EMT) in mesothelial cells (MCs).

Keywords: Caveolin; Cell height; Cell morphology; Cell motility; ERK; Mesothelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caveolin 1 / metabolism*
  • Cell Culture Techniques
  • Cell Movement
  • Cell Shape
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism
  • Epithelial-Mesenchymal Transition
  • Extracellular Signal-Regulated MAP Kinases / metabolism*
  • Mice
  • Peritoneal Cavity
  • Protein Binding
  • Signal Transduction*

Substances

  • Caveolin 1
  • Extracellular Signal-Regulated MAP Kinases