Background and aims: miR-21 has been demonstrated to play an important role in tumour progression. The aim of the present study was to analyse the correlation between miR-21 expression level and clinicopathologic features, as well as to assess the prognostic significance of miR-21 in osteosarcoma.
Methods: Eighty-four pairs of osteosarcoma and corresponding non-cancerous bone tissues were obtained, and miR-21 expression levels were detected using quantitative real-time PCR (qRT-PCR). A χ2 test was used to assess the relationship between miR-21 expression and clinicopathological features. Overall survival (OS) and disease-free survival (DFS) rates were determined by the Kaplan-Meier method and analysed by the log-rank test. The Cox proportional hazards model was used for multivariate analysis.
Results: qRT-PCR indicated that miR-21 expression in tumour tissues was strongly elevated compared with the adjacent corresponding non-cancerous bone tissue (7.88 ± 1.04 vs. 1.12 ± 0.37, respectively; P < 0.001). High miR-21 expression levels were linked to advanced clinical stage (P = 0.001), distant metastasis (P = 0.001), high tumour grade (P = 0.032) and large-sized tumours (P = 0.013). A higher miR-21 expression was significantly linked to shorter OS and DFS (both P < 0.001). Furthermore, a multivariate analysis confirmed that miR-21 was an independent and significant prognostic factor to predict poor OS and DFS (both P < 0.001).
Conclusions: Upregulation of miR-21 was associated with poor clinicopathological characteristics. It is used as a marker of poor prognosis in patients with osteosarcoma.
Keywords: Osteosarcoma; miR-21; prognosis.