Aim: There is urgent need to develop a new protocol for the evaluation of chemical substances to potentially interact with the endocrine system and induce numerous pathological issues. The recently validated in vitro screening assay is limited on monitoring two steroid hormones. Methodology & results: The H295R model cell was exposed to seven endocrine disrupting chemicals (EDCs). The levels of 17 steroid hormones in cell extracts were subsequently determined by a quantitative targeted GC/MS/MS method. Through wide coverage, this system managed to capture the effects of exposure to increasing EDCs concentrations in the entire steroidogenic pathways.
Conclusion: The developed approach could be beneficial for the mechanistic investigation of EDCs.
Keywords: H295R cells; endocrine disrupting chemicals; gas chromatography tandem mass spectrometry; steroid profiling; steroidogenesis.