Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor

Tsukuru Minamiki; Tsuyoshi Minami; Yui Sasaki; Shin-Ichi Wakida; Ryoji Kurita; Osamu Niwa; Shizuo Tokito

doi:10.3390/s16122033

Label-Free Detection of Human Glycoprotein (CgA) Using an Extended-Gated Organic Transistor-Based Immunosensor

Sensors (Basel). 2016 Nov 30;16(12):2033. doi: 10.3390/s16122033.

Authors

Tsukuru Minamiki^{1

2

3}, Tsuyoshi Minami^{4

5}, Yui Sasaki^{6

7}, Shin-Ichi Wakida⁸, Ryoji Kurita⁹, Osamu Niwa¹⁰, Shizuo Tokito¹¹

Affiliations

¹ Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. tey14898@st.yamagata-u.ac.jp.
² Japan Society for the Promotion of Science (JSPS), Ichibancho, Chiyoda-ku, Tokyo 102-8471, Japan. tey14898@st.yamagata-u.ac.jp.
³ Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. tey14898@st.yamagata-u.ac.jp.
⁴ Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. tminami@iis.u-tokyo.ac.jp.
⁵ Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. tminami@iis.u-tokyo.ac.jp.
⁶ Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. txe37183@st.yamagata-u.ac.jp.
⁷ Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan. txe37183@st.yamagata-u.ac.jp.
⁸ Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan. s.wakida@aist.go.jp.
⁹ Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. r.kurita@aist.go.jp.
¹⁰ Advanced Science Research Laboratory, Saitama Institute of Technology, Fukaya, Saitama 369-0293, Japan. niwa@sit.ac.jp.
¹¹ Research Center for Organic Electronics, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Jonan, Yonezawa, Yamagata 992-8510, Japan. tokito@yz.yamagata-u.ac.jp.

Abstract

Herein, we report on the fabrication of an extended-gated organic field-effect transistor (OFET)-based immunosensor and its application in the detection of human chromogranin A (hCgA). The fabricated OFET device possesses an extended-gate electrode immobilized with an anti-CgA antibody. The titration results of hCgA showed that the electrical changes in the OFET characteristics corresponded to the glycoprotein recognition ability of the monoclonal antibody (anti-CgA). The observed sensitivity (detection limit: 0.11 µg/mL) and selectivity indicate that the OFET-based immunosensor can be potentially applied to the rapid detection of the glycoprotein concentration without any labeling.

Keywords: antibody immobilization; chromogranin A; extended-gate; glycoprotein; immunosensor; label-free; organic field-effect transistor; self-assembled monolayer.

MeSH terms

Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / immunology
Biosensing Techniques / methods*
Chromogranin A / chemistry
Chromogranin A / immunology
Glycoproteins / analysis*
Glycoproteins / chemistry
Humans
Organic Chemicals / chemistry*
Transistors, Electronic*

Substances

Antibodies, Monoclonal
Chromogranin A
Glycoproteins
Organic Chemicals