Effects of allitridum on the transient outward potassium current in rats with heart failure

Xiao-Jing Zhao; Kun Lin; Yu Zhang; Bin Xu; Li Liu; Yi-Cheng Fu; Xi Chen; Zhong-Qi Cai; Zhi-Juan Wu; Yun Huang; Yang Li

doi:10.11909/j.issn.1671-5411.2016.09.005

Effects of allitridum on the transient outward potassium current in rats with heart failure

J Geriatr Cardiol. 2016 Sep;13(9):783-788. doi: 10.11909/j.issn.1671-5411.2016.09.005.

Authors

Xiao-Jing Zhao¹, Kun Lin¹, Yu Zhang¹, Bin Xu¹, Li Liu¹, Yi-Cheng Fu¹, Xi Chen¹, Zhong-Qi Cai¹, Zhi-Juan Wu¹, Yun Huang², Yang Li¹

Affiliations

¹ Department of Cardiology, Chinese PLA General Hospital, Beijing, China.
² Department of Gerontology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

PMID: 27899943
PMCID: PMC5122504
DOI: 10.11909/j.issn.1671-5411.2016.09.005

Abstract

Objective: To study the effect of allitridum on the transient outward potassium current (I_to) of ventricular myocytes in heart failure (HF).

Methods: The dual enzymatic method was used to separate single ventricular myocytes from Sprague Dawley rats. Patch-clamping was used to record I_to and analyze the effect of allitridum on the current.

Results: The I_to current had a significant decrease in the HF group, compared with the control group. The density of I_to in the HF group was increased after treatment of allitridum (30 µmol/L). The peak current densities of I_to were enhanced in the HF group from 6.01 ± 0.30 pA/pF to 8.41 ± 0.54 pA/pF (P < 0.01) at +50 mV after treatment with allitridum (30 µmol/L). We also determined the effect of allitridum on the gating mechanism of the I_to in the HF group.

Conclusions: We found that allitridum increased the I_to by accelerating the activation of channels and shortened the time constants of inactivation, and allitridum decreased the remodeling of I_to in ventricular myocytes of rats with HF.

Keywords: Allitridum; Heart failure; Ion current; Ventricular myocyte.