Eradication of Helicobacter pylori Infection Restores ki67, p53, and Cyclin D1 Immunoreactivity in the Human Gastric Epithelium

Konstantinos Triantafyllou; Vasilios Papadopoulos; Theodoros Emanouil; Paraskevas Gkolfakis; Vasileia Damaskou; Georgios Tziatzios; Ioannis G Panayiotides; Irene Vafiadis; Spiros D Ladas

doi:10.4137/CGast.S38330

Eradication of Helicobacter pylori Infection Restores ki67, p53, and Cyclin D1 Immunoreactivity in the Human Gastric Epithelium

Clin Med Insights Gastroenterol. 2016 Nov 17:9:73-78. doi: 10.4137/CGast.S38330. eCollection 2016.

Authors

Konstantinos Triantafyllou¹, Vasilios Papadopoulos¹, Theodoros Emanouil¹, Paraskevas Gkolfakis¹, Vasileia Damaskou², Georgios Tziatzios¹, Ioannis G Panayiotides², Irene Vafiadis³, Spiros D Ladas³

Affiliations

¹ Hepatogastroenterology Unit, Second Department of Internal Medicine-Propaedeutic, Research Institute and Diabetes Center, Medical School, National and Kapodistrian University of Athens, Attikon University General Hospital, Athens, Greece.
² Second Department of Pathology Medical School, National and Kapodistrian University of Athens, Attikon University General Hospital, Athens, Greece.
³ Academic Department of Gastroenterology, Medical School, National and Kapodistrian University of Athens, Laiko General Hospital, Athens, Greece.

Abstract

Introduction: We evaluated the effect of Helicobacter pylori (HP) eradication on p53, cyclin D1 expression, and cell proliferation in gastric mucosa.

Materials and methods: We assessed p53, cyclin D1, and ki67 immunoexpression in gastric mucosa from 31 HP chronic gastritis patients and 12 controls. Reassessment was performed 6 months after successful HP eradication.

Results: Successful eradication resulted in significant decrease of p53 (1.53 ± 0.16 vs 0.83 ± 0.19, P = 0.01) and ki67 (9.84 ± 0.96 vs 4.77 ± 0.27, P < 0.001) staining in the antrum. Similarly, p53 immunoreactivity significantly decreased in the corpus (1.27 ± 0.20 vs 0.46 ± 0.15, P = 0.02), while there was a trend for decreased corpus cyclin D1 and ki67 expression (0.17 ± 0.07 vs 0.0, P = 0.08 and 8.71 ± 1.24 vs 5.85 ± 0.54, P = 0.09, respectively). Importantly, after successful HP eradication, the immunoreactivity of the studied parameters was similar to that of controls.

Conclusion: Successful HP infection eradication restores p53, cyclin D1, and ki67 immunoreactivity in the gastric mucosa to the level of controls.

Keywords: Helicobacter pylori; cyclin D1; eradication; ki67; p53.