Development of a novel engineered antibody targeting Neisseria species

Chih-Yueh Liu; Chang-Ching Weng; Chih-Hsiang Lin; Chiou-Ying Yang; Kwok-Kong Tony Mong; Yaw-Kuen Li

doi:10.1007/s10529-016-2258-1

Development of a novel engineered antibody targeting Neisseria species

Biotechnol Lett. 2017 Mar;39(3):407-413. doi: 10.1007/s10529-016-2258-1. Epub 2016 Nov 25.

Authors

Chih-Yueh Liu¹, Chang-Ching Weng¹, Chih-Hsiang Lin¹, Chiou-Ying Yang², Kwok-Kong Tony Mong¹, Yaw-Kuen Li³

Affiliations

¹ Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 30010, Taiwan.
² Institute of Molecular Biology, National Chung Hsing University, Taichung, 40227, Taiwan.
³ Department of Applied Chemistry, National Chiao Tung University, Hsinchu, 30010, Taiwan. ykl@cc.nctu.edu.tw.

PMID: 27888365
DOI: 10.1007/s10529-016-2258-1

Abstract

Objectives: A Neissaria bacterial pilus sugar, bacillosamine, was synthesized and, for the first time, used as a probe to screen a single-chain variable fragment (scFv).

Results: Four Neisseria, Neisseria gonorrhoeae, Neisseria meningitidis, Neisseria sicca and Neisseria subflava, and two negative controls, Streptococcus pneumoniae and Escherichia coli, were tested through ELISA, immunostaining and gold nanoparticle immunological assay. All results indicated that the selected scFv is feasible for the specific detection of Neisseria species via the recognition of bacillosamine.

Conclusions: The recombinant scFv could detect Neisseria strains at 10⁶ CFU/ml.

Keywords: Bacillosamine; Neisseria gonorrhoeae; Neisseria meningitidis; Single-chain variable fragment.

MeSH terms

Antibodies, Bacterial / immunology*
Antibody Specificity / immunology
Electrophoresis, Polyacrylamide Gel
Gold / chemistry
Nanoparticles / chemistry
Neisseria / immunology*
Protein Engineering / methods*
Single-Chain Antibodies / metabolism
Species Specificity

Substances

Antibodies, Bacterial
Single-Chain Antibodies
Gold