The partitioned n-hexane, CHCl₃, and EtOAc extracts from the crude MeOH extract of Phaius mishmensis showed considerable cytotoxicities against the human breast carcinoma (MCF-7), lung carcinoma (NCI-H460), and central nervous system carcinoma (SF-268) cell lines. Four new compounds, phaindole (1), (7'R,8'R)-phaithrene (2), methyl 3-hydroxy-4,5-dimethoxypropiophenone (3), and methyl hematinate (4), as well as 44 known compounds were isolated from the MeOH extract of Phaius mishmensis. The structures of the compounds were determined using spectroscopic methods.
Keywords: (7′R,8′R)-phaithrene; Orchidaceae; Phaius mishmensis; methyl 3-hydroxy-4,5-dimethoxypropiophenone; methyl hematinate; phaindole.