Murine transgenic iPS cell line for monitoring and selection of cardiomyocytes

Stem Cell Res. 2016 Sep;17(2):266-272. doi: 10.1016/j.scr.2016.07.007. Epub 2016 Jul 21.

Abstract

We report here a transgenic murine induced pluripotent stem cell (iPSC) line expressing puromycin N-acetyltransferase (PAC) and enhanced green fluorescent protein (EGFP) under the control of α-myosin heavy chain promoter. This transgenic cell line reproducibly differentiates into EGFP-expressing cardiomyocytes (CMs) which can be generated at high purity with puromycin treatment and exhibit molecular and functional properties of immature heart muscle cells. This genetically modified iPSC line can be used for assessment of the utility of CMs for myocardial repair, pharmacological and toxicological applications and development of improved cardiac differentiation protocols.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / drug effects
  • Animals
  • Calcium Channels, L-Type / metabolism
  • Carbachol / pharmacology
  • Cell Differentiation / drug effects
  • Cell Line
  • Cell Separation / methods*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / metabolism
  • Isoproterenol / pharmacology
  • Mice
  • Mice, Transgenic
  • Microscopy, Fluorescence
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / physiology
  • Myosin Heavy Chains / genetics
  • Patch-Clamp Techniques
  • Puromycin / pharmacology
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Calcium Channels, L-Type
  • Transcription Factors
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins
  • Puromycin
  • Carbachol
  • Myosin Heavy Chains
  • Isoproterenol